Anti-AATF/Rb-binding protein Che-1

Code: ABC953 D2-231

Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Western Blotting Analysis: A representative lot detected AATF/Rb-bindi...


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€396.00 EACH
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Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in U2OS, T98G, LoVo, HT29, MCF7, HeLa, LIPARI, Jurkat, and U2OS cells were immunoprecipitated with anti-Rb (Fanciulli, M., et al. (2000). FASEB. 14:904-912).
Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, human lymphoblastoid AHH1 cells, and CV1576 lymphoblastoid cells (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in NIH 3T3 transfected cells, Synchronized NIH 3T3, HFF human fibroblasts, and HEK293 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, AT fibroblasts, NIH 3T3 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).
Immunocytochemistry Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, human lymphoblastoid AHH1 cells, and CV1576 lymphoblastoid cells (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in NIH 3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in NIH 3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in Synchronized NIH 3T3, HFF human fibroblasts, HEK293, Rb transfected HEK293 cells, and Che-1 transfected NIH3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).

This Anti-AATF antibody is validated for use in WB, IC, IP, ChIP for the detection of AATF.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

AATF/Rb-binding protein Che-1, also known as Protein Apoptosis-antagonizing transcription factor (Protein AATF), Rb-binding protein Che-1, and encoded by the gene name AATF/ CHE1/ DED/ HSPC277, is a member of the AATF family. Recent studies have indicated that AATF may function as a general inhibitor of the histone deacetylase HDAC1. AATF binds to the pocket region of RB1, and consequently it may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. In addition, AATF antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date. AATF/Rb-binding protein Che-1 is expressed at high levels in brain, heart, kidney, placenta and thymus.

Immunogen

Epitope: POLR2J domain

Linear peptide corresponding to the POLR2J domain of human AATF/Rb-binding protein Che-1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected AATF/Rb-binding protein Che-1 in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~70 kDa observed. The calculated molecular weight is 63 kDa, however AATF/Rb-binding protein Che-1 has been shown as a ~70 kDa band in western blots (Guo, G., et al. (2004). J. Biol. Chem. 279:4596-4603).

antibody formserum
antibody product typeprimary antibodies
biological sourcerabbit
clonepolyclonal
Gene Informationhuman ... AATF(26574)
NCBI accession no.NP_036270
Quality Level100
shipped indry ice
species reactivityhuman
technique(s)immunocytochemistry: suitable, western blot: suitable, ChIP: suitable, immunoprecipitation (IP): suitable
UniProt accession no.Q9NY61
This product has met the following criteria to qualify for the following awards:



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